LEADER 00000cam 2200409 a 4500 001 0717802866 003 UkLCURL 008 110222s2011 nyu 001 0 eng d 020 9781493956579 020 9781617790799 020 1617790796 035 (OCoLC)703374718 035 (UkOxU)17802866 040 YDXCP|cYDXCP|dZCH|dCUS|dGZM|dCDX|dSCB 049 |jCU|k0717802866|lo 050 4 RC 267 C2 245 00 Cancer cell culture :|bmethods and protocols /|cedited by Ian A. Cree. 250 2nd ed. 260 New York :|bHumana Press,|cc2011. 300 xiv, 502 p. :|bill. (some col.) ;|c27 cm. 440 0 Methods in molecular biology (Clifton, N.J.) ;|v731 490 1 Springer protocols 505 0 Xenotransplantation of breast cancers -- Feeder layers: Co -culture with nonneoplastic cells -- Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay -- Transfection and DNA-mediated gene transfer -- Analysis of drug interactions -- Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy -- Proteomic evaluation of cancer cells: Identification of cell surface proteins -- Measuring gene expression from cell cultures by quantitative reverse- transcriptase polymerase chain reaction -- Expression analysis of homeobox genes in leukemia/lymphoma cell lines -- Flow cytometric DNA analysis of human cancers and cell lines -- Angiogenesis assays -- Cell migration and invasion assays -- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR -- 505 0 Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay -- Purification of annexin V and its use in the detection of apoptotic cells -- Real-time cytotoxicity assays -- Differential staining cytotoxicity assay: A review -- Cell sensitivity assays: The ATP-based tumor chemosensitivity assay -- Cell sensitivity assays: The MTT assay -- Cellular chemosensitivity assays: An overview -- Isolation of endothelial cells from human tumors -- Isolation of inflammatory cells from human tumours -- Establishment and culture of leukemia-lymphoma cell lines -- Isolation and culture of ovarian cancer cells and cell lines -- Isolation and culture of squamous cell carcinoma lines -- Isolation and culture of melanoma and naevus cells and cell lines -- Isolation and culture of colon cancer cells and cell lines -- Generation of lung cancer cell line variants by drug selection or cloning -- Quality assurance and good laboratory practice -- 505 0 Elimination of mycoplasmas from infected cell lines using antibiotics -- Detecting mycoplasma contamination in cell cultures by polymerase chain reaction -- Cell culture contamination -- Cytogenetic analysis of cancer cell lines -- Online verification of human cell line identity by STRDNA typing -- Characterization and authentication of cancer cell lines: An overview -- Storage of cell lines -- Principles of cancer cell culture -- Cancer biology. 650 0 Cancer cells|xMotility|vLaboratory manuals. 700 1 Cree, Ian A. 830 0 Springer protocols (Series)
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