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Title PCR technology [electronic resource] : current innovations.
Publication Info Boca Raton, Fla. : CRC ; London : Taylor & Francis [distributor], [2013]
Edition 3rd ed. / edited by Tania Nolan and Stephen A. Bustin.



Descript xxi, 435 pages, 12 unnumbered pages of plates : ill.
Edition 3rd ed. / edited by Tania Nolan and Stephen A. Bustin.
Note Previous ed.: published as edited by Thomas Weissensteiner, Hugh G. Griffin, Annette Griffin.
Contents Template Preparation Pre-PCR Processing Strategies; Johannes Hedman, Maria Lovenklev, Petra Wolffs, Charlotta Lofstrom, Rickard Knutsson, and Peter Radstrom Use of a Liposomal Internal Control Vehicle for Whole-Process Quality Assurance of Nucleic Acid Amplification; Einar S. Berg and Tania Nolan Reaction Components Oligonucleotide Synthesis and Purification; Yannick Fillon, Theresa Creasey, Qin Xiang, and Carlos Martinez Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction; Ernest J. Mueller Real-Time Fluorescent PCR by Labeled Primer with a Single Fluorescent Molecule; Hong Yan and Liu Xiao Kun Using Scorpion Primers for Genotyping; David Whitcombe LNA: Adding New Functionality to PCR; Sren M. Echwald, Ditte Andreasen, and Peter Mouritzen Modified dNTPs: A Toolbox for Use in PCR; Natasha Paul, Olga Adelfinskaya, Elena Hidalgo Ashrafi, Tony Le, and Sabrina Shore Instruments Thermocycler Calibration and Analytical Assay Validation; Mary Span, Marc Verblakt, and Tom Hendrikx Ultra-High-Speed PCR Instrument Development; Nick Burroughs and Emmanouil Karteris Dropletization of Bioreactions: How Single-Cell Quantitative Analyses May Improve Gene Measurement and Cancer Diagnosis; Ehsan Karimiani, Amelia Markey, and Philip Day Design, Optimization, QC, and Standardization Assay Design for Real-Time qPCR; Gregory L. Shipley Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension; Maido Remm, Kaarel Krjutskov, and Andres Metspalu Development and Use of qPCR Assays for Detection and Study of Neglected Tropical and Emerging Infectious Diseases; Ashley R. Heath, Norha Deluge, Maria Galli de Amorim, and Emmanuel Dias-Neto MIQE: Guidelines for Reliable Design and Transparent Reporting of Real-Time PCR Assays; Tania Nolan and Stephen A. Bustin Data Analysis Hypothesis-Driven Approaches to Multivariate Analysis of qPCR Data; Anders Bergkvist, Azam Sheikh Muhammad, and Peter Damaschke Experiment Design, Data Management, and Univariate Statistical Analysis of Gene-Expression Data Obtained by Real-Time Quantitative PCR; Ales Tichopad, Tzachi Bar, Malin Edling, Boel Svanberg, Tania Nolan, and Anders Bergkvist Biomarker Discovery via RT-qPCR and Bioinformatical Validation; Christiane Becker, Irmgard Riedmaier, and Michael W. Pfaffl Optimization of Quantitative Real-Time PCR for Studies in Cartilage Mechanobiology; Oto Akanji, Donald Salter, and Tina Chowdhury Applications Enriching DNA Sequences with Nucleotide Variation by Thymidine Glycosylases Combined with Suppression PCR; Xinghua Pan, Alexander E. Urban, and Sherman M. Weissman Chromosome Walking by Inverse PCR; Michael Gotesman, Selwyn A. Williams, Jorge A. Garces, and Ray H. Gavin Expression Profiling of MicroRNAs by Quantitative Real-Time PCR: The Good, the Bad, and the Ugly; Mirco Castoldi, Paul Collier, Tania Nolan, and Vladimir Benes Dye-Based High-Throughput qPCR in Microfluidic Platform BioMarkT; David Svec, Vendula Rusnakova, Vlasta Korenkova, and Mikael Kubista Surface Plasmon Resonance-Based Biosensor Technology for Real-Time Detection of PCR Products; Giordana Feriotto and Roberto Gambari Current Innovations in the Development and Application of the qPCR in a Resource-Limited Setting; Clare Watt and Jim Huggett A Review of Isothermal Nucleic Acid Amplification Technologies; Gavin J. Nixon and Claire A. Bushell Proximity Ligation Assay for Protein Quantification; David W. Ruff, Mark E. Shannon, Shiaw-Min Chen, Simon Fredriksson, and Ulf Landegren High-Resolution Melt Analysis; Einar S. Berg and Tania Nolan Index
Note 1 concurrent user. UkHlHU
ISBN 9781439848135 (e-book)
Click on the terms below to find similar items in the catalogue
Subject Polymerase chain reaction -- Methodology.
Alt author Nolan, Tania.
Bustin, Stephen A., 1954-
Weissensteiner, Thomas. PCR technology.
Descript xxi, 435 pages, 12 unnumbered pages of plates : ill.
Edition 3rd ed. / edited by Tania Nolan and Stephen A. Bustin.
Note Previous ed.: published as edited by Thomas Weissensteiner, Hugh G. Griffin, Annette Griffin.
Contents Template Preparation Pre-PCR Processing Strategies; Johannes Hedman, Maria Lovenklev, Petra Wolffs, Charlotta Lofstrom, Rickard Knutsson, and Peter Radstrom Use of a Liposomal Internal Control Vehicle for Whole-Process Quality Assurance of Nucleic Acid Amplification; Einar S. Berg and Tania Nolan Reaction Components Oligonucleotide Synthesis and Purification; Yannick Fillon, Theresa Creasey, Qin Xiang, and Carlos Martinez Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction; Ernest J. Mueller Real-Time Fluorescent PCR by Labeled Primer with a Single Fluorescent Molecule; Hong Yan and Liu Xiao Kun Using Scorpion Primers for Genotyping; David Whitcombe LNA: Adding New Functionality to PCR; Sren M. Echwald, Ditte Andreasen, and Peter Mouritzen Modified dNTPs: A Toolbox for Use in PCR; Natasha Paul, Olga Adelfinskaya, Elena Hidalgo Ashrafi, Tony Le, and Sabrina Shore Instruments Thermocycler Calibration and Analytical Assay Validation; Mary Span, Marc Verblakt, and Tom Hendrikx Ultra-High-Speed PCR Instrument Development; Nick Burroughs and Emmanouil Karteris Dropletization of Bioreactions: How Single-Cell Quantitative Analyses May Improve Gene Measurement and Cancer Diagnosis; Ehsan Karimiani, Amelia Markey, and Philip Day Design, Optimization, QC, and Standardization Assay Design for Real-Time qPCR; Gregory L. Shipley Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension; Maido Remm, Kaarel Krjutskov, and Andres Metspalu Development and Use of qPCR Assays for Detection and Study of Neglected Tropical and Emerging Infectious Diseases; Ashley R. Heath, Norha Deluge, Maria Galli de Amorim, and Emmanuel Dias-Neto MIQE: Guidelines for Reliable Design and Transparent Reporting of Real-Time PCR Assays; Tania Nolan and Stephen A. Bustin Data Analysis Hypothesis-Driven Approaches to Multivariate Analysis of qPCR Data; Anders Bergkvist, Azam Sheikh Muhammad, and Peter Damaschke Experiment Design, Data Management, and Univariate Statistical Analysis of Gene-Expression Data Obtained by Real-Time Quantitative PCR; Ales Tichopad, Tzachi Bar, Malin Edling, Boel Svanberg, Tania Nolan, and Anders Bergkvist Biomarker Discovery via RT-qPCR and Bioinformatical Validation; Christiane Becker, Irmgard Riedmaier, and Michael W. Pfaffl Optimization of Quantitative Real-Time PCR for Studies in Cartilage Mechanobiology; Oto Akanji, Donald Salter, and Tina Chowdhury Applications Enriching DNA Sequences with Nucleotide Variation by Thymidine Glycosylases Combined with Suppression PCR; Xinghua Pan, Alexander E. Urban, and Sherman M. Weissman Chromosome Walking by Inverse PCR; Michael Gotesman, Selwyn A. Williams, Jorge A. Garces, and Ray H. Gavin Expression Profiling of MicroRNAs by Quantitative Real-Time PCR: The Good, the Bad, and the Ugly; Mirco Castoldi, Paul Collier, Tania Nolan, and Vladimir Benes Dye-Based High-Throughput qPCR in Microfluidic Platform BioMarkT; David Svec, Vendula Rusnakova, Vlasta Korenkova, and Mikael Kubista Surface Plasmon Resonance-Based Biosensor Technology for Real-Time Detection of PCR Products; Giordana Feriotto and Roberto Gambari Current Innovations in the Development and Application of the qPCR in a Resource-Limited Setting; Clare Watt and Jim Huggett A Review of Isothermal Nucleic Acid Amplification Technologies; Gavin J. Nixon and Claire A. Bushell Proximity Ligation Assay for Protein Quantification; David W. Ruff, Mark E. Shannon, Shiaw-Min Chen, Simon Fredriksson, and Ulf Landegren High-Resolution Melt Analysis; Einar S. Berg and Tania Nolan Index
Note 1 concurrent user. UkHlHU
ISBN 9781439848135 (e-book)
Subject Polymerase chain reaction -- Methodology.
Alt author Nolan, Tania.
Bustin, Stephen A., 1954-
Weissensteiner, Thomas. PCR technology.

Subject Polymerase chain reaction -- Methodology.
Descript xxi, 435 pages, 12 unnumbered pages of plates : ill.
Note Previous ed.: published as edited by Thomas Weissensteiner, Hugh G. Griffin, Annette Griffin.
Contents Template Preparation Pre-PCR Processing Strategies; Johannes Hedman, Maria Lovenklev, Petra Wolffs, Charlotta Lofstrom, Rickard Knutsson, and Peter Radstrom Use of a Liposomal Internal Control Vehicle for Whole-Process Quality Assurance of Nucleic Acid Amplification; Einar S. Berg and Tania Nolan Reaction Components Oligonucleotide Synthesis and Purification; Yannick Fillon, Theresa Creasey, Qin Xiang, and Carlos Martinez Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction; Ernest J. Mueller Real-Time Fluorescent PCR by Labeled Primer with a Single Fluorescent Molecule; Hong Yan and Liu Xiao Kun Using Scorpion Primers for Genotyping; David Whitcombe LNA: Adding New Functionality to PCR; Sren M. Echwald, Ditte Andreasen, and Peter Mouritzen Modified dNTPs: A Toolbox for Use in PCR; Natasha Paul, Olga Adelfinskaya, Elena Hidalgo Ashrafi, Tony Le, and Sabrina Shore Instruments Thermocycler Calibration and Analytical Assay Validation; Mary Span, Marc Verblakt, and Tom Hendrikx Ultra-High-Speed PCR Instrument Development; Nick Burroughs and Emmanouil Karteris Dropletization of Bioreactions: How Single-Cell Quantitative Analyses May Improve Gene Measurement and Cancer Diagnosis; Ehsan Karimiani, Amelia Markey, and Philip Day Design, Optimization, QC, and Standardization Assay Design for Real-Time qPCR; Gregory L. Shipley Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension; Maido Remm, Kaarel Krjutskov, and Andres Metspalu Development and Use of qPCR Assays for Detection and Study of Neglected Tropical and Emerging Infectious Diseases; Ashley R. Heath, Norha Deluge, Maria Galli de Amorim, and Emmanuel Dias-Neto MIQE: Guidelines for Reliable Design and Transparent Reporting of Real-Time PCR Assays; Tania Nolan and Stephen A. Bustin Data Analysis Hypothesis-Driven Approaches to Multivariate Analysis of qPCR Data; Anders Bergkvist, Azam Sheikh Muhammad, and Peter Damaschke Experiment Design, Data Management, and Univariate Statistical Analysis of Gene-Expression Data Obtained by Real-Time Quantitative PCR; Ales Tichopad, Tzachi Bar, Malin Edling, Boel Svanberg, Tania Nolan, and Anders Bergkvist Biomarker Discovery via RT-qPCR and Bioinformatical Validation; Christiane Becker, Irmgard Riedmaier, and Michael W. Pfaffl Optimization of Quantitative Real-Time PCR for Studies in Cartilage Mechanobiology; Oto Akanji, Donald Salter, and Tina Chowdhury Applications Enriching DNA Sequences with Nucleotide Variation by Thymidine Glycosylases Combined with Suppression PCR; Xinghua Pan, Alexander E. Urban, and Sherman M. Weissman Chromosome Walking by Inverse PCR; Michael Gotesman, Selwyn A. Williams, Jorge A. Garces, and Ray H. Gavin Expression Profiling of MicroRNAs by Quantitative Real-Time PCR: The Good, the Bad, and the Ugly; Mirco Castoldi, Paul Collier, Tania Nolan, and Vladimir Benes Dye-Based High-Throughput qPCR in Microfluidic Platform BioMarkT; David Svec, Vendula Rusnakova, Vlasta Korenkova, and Mikael Kubista Surface Plasmon Resonance-Based Biosensor Technology for Real-Time Detection of PCR Products; Giordana Feriotto and Roberto Gambari Current Innovations in the Development and Application of the qPCR in a Resource-Limited Setting; Clare Watt and Jim Huggett A Review of Isothermal Nucleic Acid Amplification Technologies; Gavin J. Nixon and Claire A. Bushell Proximity Ligation Assay for Protein Quantification; David W. Ruff, Mark E. Shannon, Shiaw-Min Chen, Simon Fredriksson, and Ulf Landegren High-Resolution Melt Analysis; Einar S. Berg and Tania Nolan Index
Note 1 concurrent user. UkHlHU
Alt author Nolan, Tania.
Bustin, Stephen A., 1954-
Weissensteiner, Thomas. PCR technology.
ISBN 9781439848135 (e-book)

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